Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model

ABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans.

Existe diferença entre o estado nutricional de pacientes com doença pulmonar obstrutiva crônica e outras doenças pulmonares? / Is there a difference between the nutritional status of patients with chronic obstructive pulmonary disease and other lung diseases?

Introdução: A doença pulmonar obstrutiva crônica (DPOC) é caracterizada pela lenta e progressiva obstrução crônica do fluxo aéreo, pouco reversível, associada a uma resposta inflamatória anormal dos pulmões e inalação de partículas ou gases tóxicos. Objetivo: Comparar o estado nutricional de pacientes hospitalizados com diagnóstico clínico de DPOC com outras doenças pulmonares. Método: Foram utilizados prontuários com dados referentes às características antropométricas, clínicas e alimentares de pacientes com diagnóstico clínico de DPOC e de outras doenças pulmonares. Para associar os dois grupos de pacientes, foram aplicados os testes T de Student, exato de Fisher e Mann-Whitney conforme a distribuição de normalidade. Resultados: Participaram do estudo 76 pacientes, não havendo diferença estatística entre ambos os grupos quanto à ingestão alimentar (p>0,05). Os pacientes com DPOC apresentaram mais desnutrição, porém sem significância (p>0,05) e a prega cutânea tricipital foi menor entre os pacientes com DPOC (p<0,05). Conclusões: Não há diferença entre o estado nutricional e dietético de pacientes com DPOC e demais doenças pulmonares.(AU)

Introduction: Chronic obstructive pulmonary disease (COPD) are characterized by slow and progressive chronic airflow obstruction, not easy reversible, associated with an abnormal inflammatory response of the lungs and inhalation of toxic particles or gases. Objective: To compare the nutritional status of hospitalized patients with clinical diagnosis of COPD with other pulmonary diseases. Methods: Documents of data regarding the anthropometric, clinical and dietary characteristics of patients with clinical diagnosis of COPD and other pulmonary diseases was used. In order to associate the two groups of patients, T Student, Fisher's exact and Mann-Whitney tests was applied according to the distribution of normality. Results: 76 patients participated, and there was no statistical difference between the two groups regarding food intake (p>0.05), patients with COPD have shown more malnutrition, but without significance (p>0.05) and triceps tick up was lower among the patients with COPD (p<0.05). Conclusions: There is no difference between the nutritional status of patients with COPD and other pulmonary diseases.(AU)